General Cell Based Assays
Meso Scale Discovery U-PLEX Human MCP-1 ASY 25 PL
The Meso Scale Discovery U-PLEX Human MCP-1 Assay Kit quantifies monocyte chemoattractant protein 1 (MCP-1) in human serum plasma and cell culture supernatants Using MSD s SULFO-TAG labels and multi-array technology it measures MCP-1 levels down to 0 4 pg/mL The kit includes a 96-well plate with capture antibodies detection antibodies buffers blockers calibrators and controls The assay takes about 3 5 hours and requires an MSD instrument like the MESO QuickPlex SQ 120 It offers high sensitivity broad range and minimal sample volume for efficient MCP-1 analysis
ILEX LIFE SCIENCES LLC QuickZyme Sensitive Tissue Hydroxyproline Kit, 2 x 96 Assays, 96-well plate format, colorimetric assay for low level collagen analysis (tissue, tissue homogenates samples)
QuickZyme Sensitive Tissue Hydroxyproline Assay Kit enables collagen analysis in small tissue samples with low collagen levels. Quantitative colorimetric determination of hydroxyproline residues, obtained by acid hydrolysis of collagen. The only hydroxyproline-based assay which shows no matrix effect and no need for further dilution of hydrolysate. No drying of hydrolysate is needed, resulting in a fast and easy (96-well plate format) assay. NOTE: Kit does not contain tubes and protocol for collagen hydrolysis. If you need these items please order the QuickZyme Sensitive Tissue Collagen Assay. Application: tissues with low collagen/hydroxyproline content (ex. liver). Sensitivity: 2.4 µM (0.3 µg/ml). Assay-time: < 2 hours. Collagen is a main component of extracellular matrix. Dysregulation in collagen production results in pathologies such as fibrosis (too much collagen), or osteoarthritis (too little collagen). Thus measurement of collagen production is important in many studies.
Abcam Glutamate Assay Kit (Fluorometric)
Glutamate Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress CDC25A Degradation Assay, 2-Plate Kit. (93-0765E3CP0M)
The PathHunter eXpress CDC25A Degradation Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting the cell cycle checkpoint pathway. This kit enables detection of levels of CDC25a, allowing for assessment of CHK-1-activation-induced CDC25a degradation. This product uses EFC technology. The cell line included overexpresses CDC25a target protein fused to ProLabel. EA is contained in the detection reagent. Upon pathway-activation-induced degradation of CDC25a EFC signal is lost. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter BDKRB2 Bioassay Kit, 2-Plate. (93-0189Y2-00113)
The PathHunter BDKRB2 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. bradykinin) based activation of BDKRB2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged BDKRB2 and EA-tagged β-Arrestin-2. Activation of BDKRB2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Meso Scale Discovery R-PLEX Human IL-24 ASY
The R-PLEX Human IL-24 Assay QuickPlex 5-PL Kit from Meso Scale Diagnostics is a multiplex immunoassay kit for the quantitative measurement of human Interleukin 24 (IL-24) in serum plasma and cell culture supernatants The kit utilizes electrochemiluminescence (ECL) detection technology and provides reagents for 5-plex analysis of IL-24 in a quick and streamlined workflow The assay has high sensitivity wide dynamic range and requires low sample volume This product can aid in cytokine profiling and research related to cancer and inflammatory conditions
Eurofins Discoverx PathHunter eXpress HCRTR2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0427E2CP0L)
The PathHunter HCRTR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. orexin A) based activation of HCRTR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HCRTR2 and EA-tagged β-Arrestin-2. Activation of HCRTR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Human Phospho-Phospholamban (Thr17) Detection Kit, Control Lysate
HTRF Human Phospho-Phospholamban (Thr17) Detection Kit, Control Lysate
Immunology Consultants Recombinant Hamster (CHO) Lysosomal Protective Protein, 0.025 mg, Lyophilized
This recombinant CHO Lysosomal Protective Protein (CTSA; UniProt G3H8V5) is expressed in CHO‑S cells with a 6xHIS tag and purified by nickel affinity chromatography to >95% purity. Under reducing conditions, CTSA appears at ~63 kDa and is quantified by Bradford assay and A280. CTSA is a key lysosomal enzyme involved in protein degradation and stabilization of other hydrolases, making it a relevant CHO‑derived impurity in biologics manufacturing. This high‑quality recombinant antigen provides a consistent, biologically relevant standard for ELISA development, HCP assay calibration, and impurity profiling. Its defined composition supports accurate analytical method development and bioprocess monitoring. Ideal for teams studying lysosomal enzymes, CHO‑derived contaminants, or protease‑related risks in therapeutic protein production.
Eurofins Discoverx cAMP Hunter eXpress TACR3 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0092E2CP2M)
The cAMP Hunter TACR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. senktide, neurokinins) based activation of TACR3 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type TACR3, a naturally Gs coupled GPCR. Upon activation of TACR3, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Sigma Aldrich Fine Chemicals Biosciences fluorometric Intracellular
Reactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels ROS contribute to cell signaling and host defense. Increased ROS generation above the detoxification capacity of the biological system results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids essential proteins and DNA. ROS has been implicated in disease states such as Alzheimer disease Parkinson disease cancer and aging.
Eurofins Discoverx PathHunter eXpress MC4R U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0211E3CP0L)
The PathHunter MC4R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. α-MSH) based activation of MC4R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MC4R and EA-tagged β-Arrestin-2. Activation of MC4R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Human IL-17D ASY
The Meso Scale Discovery U-PLEX Human IL-17D Assay Kit is a multiplex immunoassay that measures IL-17D levels in human serum plasma and cell culture supernatant samples The assay uses electrochemiluminescence detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This assay kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with electrochemiluminescent labels calibrators and diluents needed to perform the assay The kit can quantitatively measure IL-17D levels with high precision and accuracy It is for research use only
Eurofins Discoverx cAMP Hunter eXpress ADORA2B CHO-K1 GPCR Assay, 10-Plate Kit. (95-0041E2CP2L)
The cAMP Hunter ADORA2B eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. NECA) based activation of ADORA2B GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADORA2B, a naturally Gαs-coupled GPCR. Upon activation of ADORA2B, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mCCR3 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0522E2MCP0M)
The PathHunter mCCR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL24) based activation of mCCR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCCR3 and EA-tagged β-Arrestin-2. Activation of mCCR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.